Confocal Excitation


The light source for fluorescence excitation in confocal microscopes is a laser or a combination of lasers with varying color. Lasers are used for technical reasons [2]. Unfortunately, traditional lasers emit usually only a single wavelength, or a few lines only (a modern solution is the white light laser [3]). However, the fluorochromes used in biomedical imaging show excitation bands anywhere in the spectrum between near UV and near



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