Month: October 2019

Chat with Dr. Thomas Mathivet about In Vivo Cardiovascular Imaging of the Brain

Dr. Alvarez: Hello Thomas, we are very happy to have you here in Mannheim at Leica (Microsystems) and perhaps you can tell us a little bit about yourself in two or three sentences and your research interests? Dr. Mathivet: So, I am a researcher in France at the Cardiovascular Institute of Paris looking to understand …

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Pore Scale Visualization of Drainage in 3D Porous Media by Confocal Microscopy

Porous media infiltration is dictated by the interplay between capillary and viscous forces and is, therefore, highly dependent on the flow conditions and the ratio of viscosity to surface tension (capillary number, Ca). The authors used fast confocal microscopy and fluorescent dyes to visualize the dynamics of liquid displacement in wet pores infiltrated by a …

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10 Moments in History That Changed Cameras

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Ultrastructural Preservation and Improved Visualization of Membranes in Primary Bovine Chromaffin Cells

Fresh adrenal glands derived from adult bovine were digested with Locke’s solution containing collagenase P, trypsin inhibitor and bovine albumin serum. The glands were cut longitudinally to expose the digested medulla. The medulla was minced in Locke’s solution and filtered through a 250 μm nylon mesh. The filtrate was centrifuged at 50 g for 4 …

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SP8 FALCON: a novel concept in fluorescence lifetime imaging enabling video-rate confocal FLIM

Confocal imaging is the current standard for fluorescence imaging in the life sciences. Functional imaging goes beyond the traditional recording of the location and concentration of molecular species, and enables further investigation of molecular function: their interactions with other biomolecules as well as their activity, conformation, molecular environment and post translational modifications. Ideally, this must be …

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3-Dimensional Imaging of Macroscopic Defects in Aluminum Alloys

The investigation of macroscale defects in aluminum (Al) alloys with a rapid 3-dimensional (3D) imaging approach is described in this report. Aluminum (Al) alloys play an important role in the production of aircraft and vehicles, as well as products in other industries. Defects present in the Al alloy used for the production of aircraft, vehicles, …

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Studying the Microstructure of Natural Polymers in Fine Detail

The microstructure of polymers controls their chemical reactivity as well as their mechanical and transport properties. However, quantitative characterization of the microstructure of soft polymers is not often possible or practical with the few analytical methods available to study it at the sub-micrometer scale. Freeze-fracturing is one such method, but often the surfaces of samples …

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