Month: March 2017

Work Efficiently in Developmental Biology and Medical Research with Stereo Microscopy: Rodent and Small Animal Surgery

Mammary gland development occurs over multiple stages [37]. At birth, only a rudimentary milk duct tree is present. Hormones induce communication between the mammary epithelial and mesenchymal cells and regulate their development. During puberty, this regulation leads to mammary terminal end bud cells, eventually reaching the “fat pad” at which point they begin to form …

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Full-HD Image Injection Provides a Complete Picture of the Surgical Site Directly in the Microscope Eyepieces

The human skull is an especially delicate area for surgery. To differentiate healthy tissue from tumor cells, or treat an aneurysm for example, the surgeon needs a complete picture of the surgical area. Supplementary information provided by Image Guided Surgery (IGS) or neuro navigation systems, endoscope, and in some cases fluorescence, can support the surgeon’s …

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Navigating Through the Brain

One of the challenges of neurosurgery is orientation at the surgical site. When resecting tumors, removing arteriovenous malformations, or clipping aneurysms, surgeons often have to work near healthy and functional brain tissue. When resecting the tumor, the challenge is always to spare as much healthy tissue as possible. Neuronavigation technology, also referred to as Image …

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Sylvian dissection with ICG, viewed with FL800 fluorescence

In vascular and neoplastic surgery sylvian dissection is an essential skill. The neurosurgeon needs to recognize the superficial sylvian vein (SSV) in order to safely and accurately achieve a wide opening of the sylvian fissure.   A report by Dr. Hisashi Kubota and his colleagues at the Department of Neurosurgery, Kindai University Faculty of Medicine, …

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Micro-CT of Insect Larva Protocol

IntroductionSpecies: red blood worm (midge larva)Critical point drying of midge larvae with subsequent X-ray micro-computed tomography (micro-CT) to reconst­ruct the inner anatomy. ProcedureSample Holder:Sample was placed individually in the chambers of Arthropoda holder. Fixation and Dehydration 2.5 % Glutardialdehyde (in 0.1 M phosphate buffer) overnight 0.1 M phosphate buffer (1.8% Sucrose, pH 7.2) 3×10 min. …

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Milestones in Incident Light Fluorescence Microscopy

Considering these facts it was now possible to use the advantages of epi-illumination by applying narrow band excitation with blue and green, powered with ordinary high-pressure mercury arc lamps. This enhancement satisfied the upcoming demand for fluorescence microscopy in life sciences and medicine.  Out of Ploem’s inventions, Leitz designed a new multi-wavelength fluorescence epi-illuminator …

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