When to Watermark
Make a beautiful portfolio or website: http://squarespace.com/tony, coupon code ‘portfolio’ Source link
Month: January 2017
Variety of RNAs in Peripheral Blood Cells, Plasma, and Plasma Fractions
Human peripheral blood contains RNA in cells and in extracellular membrane vesicles, microvesicles and exosomes, as well as in cell-free ribonucleoproteins. Circulating mRNAs and noncoding RNAs, being internalized, possess the ability to modulate vital processes in recipient cells. In this study, with SOLiD sequencing technology, we performed identification, classification, and quantification of RNAs from blood …
Variety of RNAs in Peripheral Blood Cells, Plasma, and Plasma Fractions Read More »
Drosophila larvae – Sample Preparation for Cryo-SEM
Drosophila larvae were sandwiched between two 3 mm aluminum slit carriers with the 100 µm cavities facin each other and high-pressure frozen with a Leica EM HPM100. No ethanol as synchronization media was used, 1-hexadecene was used as filler. The wholes of the slit carriers were filled with filter tips dipped in 1-hexadecene to keep …
Drosophila larvae – Sample Preparation for Cryo-SEM Read More »
Giardia lamblia – Sample Preparation for Cryo-SEM
A 100 mesh copper grid (12 um thickness) was dipped into a concentrated Giardia suspension and sandwiched between two flat 3 mm aluminum specimen carriers with scratched surfaces. Subsequently, the sandwich was transferred to the widened hole of a middle plate (3.1 mm diameter). A 50 um spacer ring was added on top and the …
Giardia lamblia – Sample Preparation for Cryo-SEM Read More »
Clarifying Tissue Clearing
Biological specimens are intrinsically three dimensional; however because of the obscuring effects of light scatter, imaging deep into a tissue volume is problematic. Although efforts to eliminate the scatter by “clearing” the tissue have been ongoing for over a century, there have been a large number of recent innovations. This review introduces the physical basis …
The Bif-1-Dynamin 2 Membrane Fission Machinery Regulates Atg9-Containing Vesicle Generation at the Rab11-Positive Reservoirs
Atg9 is a multispanning transmembrane protein that is required for autophagosome formation. During autophagy, vesicles containing Atg9 are generated through an unknown mechanism and delivered to the autophagosome formation sites. We have previously reported that Atg9-containing membranes undergo continuous tubulation and fission during nutrient starvation in a manner dependent on the curvature-inducing protein Bif-1/Sh3glb1. Here, …
Cross Sectioning of Cadmiumsulphide (CdS) for Cathodoluminescence
Cathodoluminescence can be used to achieve spectra and high resolution images of impurity and structural defects in semicondoctors, minerals and insulating materials. This application note explains how to prepare a perfect sample surface for carhodoluminescence and how to use ion beam slope cutting to prepare the sample surface free of any preparation artefacts. Source link
Picea abies (L.) KARST – Sample Preparation for TEM
Small sections of leaves (1mm2) were cut with a razor blade on a modelling wax plate in a drop of 3% glutaraldehyde in 0.06M Sørensen phosphate buffer at pH 7.2. Sections were then evacuated with a water jet vacuum pump for a maximum of 10 seconds in a vial filled with the above described fixative …
Picea abies (L.) KARST – Sample Preparation for TEM Read More »
Are Pro Photographers Going Extinct?
Big portrait studios are almost completely gone. Many fine art photographers can’t pay their bills. Can you still make a living doing what you love? YES! Or maybe not. We explore the future of professional photography and find that some areas are flourishing, while others are bleak. Get a free book & Audible trial at …
Cross Sectioning of Copper for Electron Backscattered Diffraction (EBSD)
Electron Backscattered Diffraction (EBSD) is a surface technique creating diffraction patterns (Kikuchi-bands). It can be used for crystal orientation mapping, defect studies, phase identification, grain boundary studies and morphological studies. The information depth is just a few nm, therefore good sample preparation is very important to avoid damages. In this application note we describe how …
Cross Sectioning of Copper for Electron Backscattered Diffraction (EBSD) Read More »