Month: November 2016

Leica History

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Multispectral Phloem-Mobile Probes: Properties and Applications

Using Arabidopsis (Arabidopsis thaliana) seedlings, we identified a range of small fluorescent probes that entered the translocation stream and were unloaded at the root tip. These probes had absorbance/emission maxima ranging from 367/454 to 546/576 nm and represent a versatile toolbox for studying phloem transport. Of the probes that we tested, naturally occurring fluorescent coumarin …

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Evaluation of Zebrafish as a Model to Study the Pathogenesis of the Opportunistic Pathogen Cronobacter Turicensis

Bacteria belonging to the genus Cronobacter spp. have been recognized as causative agents of life-threatening systemic infections, primarily in premature, low-birth weight and/or immune-compromised neonates. Knowledge remains scarce regarding the underlying molecular mechanisms of disease development. In this study, we evaluated the use of a zebrafish model to study the pathogenesis of Cronobacter turicensis LMG …

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High-Resolution Carbon Coating: How much Carbon is too much?

Multiple evaporation using adaptive carbon thread evaporation was already shown to be a benefical process for obtaining ultrathin carbon films with a uniform thickness and good mechanical stability. These films need to be supported by a holey carbon or Quantifoil grid. The protocol of the synthesis of such carbon films can be found in this …

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Each Atom Counts: Protect Your Samples Prior to FIB Processing

As an alternative, amorphous carbon coating can be used as a protective layer. Although carbon can be deposited by a range of different deposition modes, this application poses some stringent demands on the quality of the layer. In order to be suited for this application, the carbon layer should be: (1) of a uniform density, …

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Factors to Consider when Selecting Student Microscopes for Schools and Universities

What classroom will the microscopes be used in? From our experience, there are two types of classrooms: A. The lab / wet lab. Microscopes are set up once and remain at the same place all the time. There are long rows of tables, often with little space between the rows. Factor to consider:Cables: …

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Commercially Available Hand Creams – Sample Preparation for Cryo-SEM

Hand creams having different water contents were applied into the 100 μm cavities of two 3 mm type A sample carriers which were then closed cream sides inwards. The sample assembly was high pressure frozen with a Leica EM HPM100 and moved to a cooled Leica EM VCT100 loading station. The closed carriers were mounted …

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“Shallow Trench Isolation” Structures – Sample Preparation for TEM | Learn & Share

The cross-sectional preparation of structured semiconductor materials requires a very thorough mechanical preparation. In doing this, it must be ensured that the structure of interest should be located as close to the centre of the sample as possible. As the sample will be ion milled from both sides, a specific preparation of the structure is …

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