Month: October 2016

Are Camera Reviews a Waste of Time?

Are camera reviews worthwhile, or are you wasting your time thinking about gear? Chelsea & Tony bring their experience as camera reviewers to talk about the benefits of watching reviews, as well as those people who spend far too much time thinking about their gear. Our sponsor: squarespace.com/tony, coupon code ‘portfolio’ Source link

Cross-Sectional Preparation of Structured Semiconductor Materials for TEM

Double sided polishing:Leica EM TXP:Diamond foils: 15 μm, 9 μm, 6 μm, 3 μm, 1 μm and 0.5 μm at 2200 rpm until the final sample thickness of 30 μm was reached. Ion Milling  Sample holder: Quick-clamp-holder Acceleration voltage: 7 kV and 2.5 kV (final step) Milling angle: 4° (milling on both sides) Sample movement: …

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Contrast Enhancement of Polished Cross Sections of Semiconductor Structures – Sample Preparation for SEM

The surfaces of polished cross sections often show fine scratches and residues of the removed material or of the abrasive material. The artefacts are strongly material-dependent, and are mostly only detectable at higher resolutions in the scanning electron microscope. A further problem arises from the fact that the ground section mostly only has low …

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Work More Efficiently in Developmental Biology With Stereo Microscopy: Zebrafish, Medaka, and Xenopus

Transgenesis Genetic modifications in zebrafish, medaka, and xenopus are typically carried out by microinjection of DNA, RNA, or dyes (as for plasmids, mRNAs, morpholinos, siRNAs, etc.) [8]. These manipulations are efficiently supported by the optical magnifications achievable with a stereo microscope, such as the Leica M50, Leica M60, or Leica M80 [9]. If DNA is injected into a cell and …

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Initiation of Lamellipodia and Ruffles Involves Cooperation Between mDia1 and the Arp2/3 Complex

Protrusion of lamellipodia and ruffles requires polymerization of branched actin filaments by the Arp2/3 complex. Although regulation of Arp2/3 complex activity has been extensively investigated, the mechanism of initiation of lamellipodia and ruffles remains poorly understood. Here, we show that mDia1 acts in concert with the Arp2/3 complex to promote initiation of lamellipodia and ruffles. …

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Visualizing Tropoelastin in a Long-Term Human Elastic Fibre Cell Culture Model

Elastin is an essential protein found in a variety of tissues where resilience and flexibility are needed, such as the skin and the heart. When aiming to engineer suitable implants, elastic fibres are needed to allow adequate tissue renewal. However, the visualization of human elastogenesis remains in the dark. To date, the visualization of human …

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P53- and Mevalonate Pathway–Driven Malignancies Require Arf6 for Metastasis and Drug Resistance

Drug resistance, metastasis, and a mesenchymal transcriptional program are central features of aggressive breast tumors. The GTPase Arf6, often overexpressed in tumors, is critical to promote epithelial–mesenchymal transition and invasiveness. The metabolic mevalonate pathway (MVP) is associated with tumor invasiveness and known to prenylate proteins, but which prenylated proteins are critical for MVP-driven cancers is …

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