Month: September 2016

Work More Efficiently In Developmental Biology With Stereo Microscopy: Fruit Flies (Drosophila Melanogaster)

Drosophila melanogaster has been a model organism for genetic studies for more than 100 years and continues to be widely used today for developmental biological research in the areas of genetics, physiology, pathogenesis, and evolution [1, 2]. It is relatively easy and inexpensive to care for in large numbers. It breeds quickly, lays many eggs, and has …

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Ultra-thin Carbon Support Films for Improved STEM-EELS Analysis of Nanoparticles

Images 1a and 1b show the dispersion of CdSe quantum plates onto 3 nm carbon film deposited on a Quantfoil (see application note ‘Ultra-Thin Carbon Films’). The ultra-thin carbon films can also be deposited on holey carbon films, but in order to show the decreased thickness a Quantifoil is used as a reference. Imaging the …

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Ways to Reveal More from your Samples: Ultra-Thin Carbon Films

Carbon films are preferred because of their mechanical strength, conductivity and thermostability. The preparation of ultra-thin carbon films in a straightforward manner can be a laborious task owing to the non-reproducible characteristics of conventional carbon deposition methods. The structure and quality of the carbon filmes are governed by the evaporation characteristics (evaporation mode, vacuum level, …

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Measuring the 3D STED-PSF with a new Type of Fluorescent Beads

While the theoretical estimation of the PSF of the STED system is a successful approach for performing deconvolution, it is important to remember that it is still based on approximations and on an ideal, typical acquisition conditions rather than on an actual experimental situation. Predicting the STED PSF for each specific fluorophore, for each sample …

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Work Efficiently in Developmental Biology with Stereo and Confocal Microscopy: C. elegans

Transgenesis, Microinjection, and RNAi There are several ways to alter the gene/protein expression of a worm. Stable and transient gene modification is carried out with microinjections into the worm. The two main methods are: transposons [13], also known as jumping genes, or the CRISPR/Cas9 [14] system. The transgenic yield with CRISPR is lower and requires one generation …

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Cross Section of an Aluminium Sample for Electron Backscattered Diffraction (EBSD)

PurposeElectron backscattered diffraction (EBSD) is for example used to examine the crystallographic orientation of material. The sample preparetion for such samples is sometimes very tricky as the depth of information is just few nm (~20nm or less). That means the sample surface must be flat and free of preparation artefacts. Mechanical polishing leads mostly to …

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