Month: August 2016

Picture This: Photography Podcast: Annie Leibovitz

Tony and Chelsea cover the life and work of Annie Leibovitz, a portrait photographer who took the last portrait of John Lennon and toured with the Rolling Stones before becoming sober and shooting the most famous magazine covers for Vanity Fair and Vogue. Our sponsor: squarespace.com/tony, coupon code ‘portfolio’ Source link

Image Gallery: Application Options with Leica DM2500 LED Microscope

The number of applications in microscopy can be as manifold as life itself. Different specimens demand different contrast methods to get a decent result. Unstained cells normally do not show a very high contrast. Researchers approach this problem by either staining the specimen with dyes respectively fluorescent molecules; or adding contrast by manipulating the light …

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Epoxy Resin Embedding of Animal and Human Tissues for Pathological Diagnosis and Research

Ultrastructural analysisEpoxy resin embedding of different tissues e.g. animal tissues, human tumours and non-neoplastic lesions ReagentsCacodylate buffer (0.1M)Fixatives: modified Karnovsky fixative (2% paraformaldehyde + 2.5% glutaraldehyde in 0.1M cacodylate buffer); Osmium-tetroxide Os04 (1% in 0.1M cacodylate buffer)Ethanols (50%, 70%, 95%, abs.)Acetone abs. Epoxy resin (all EPON components from Electron Microscopy Sciences, Munich/Germany):EMbed 812 90g #14960DDSA …

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Mirror-Enhanced Super-Resolution Microscopy

Axial excitation confinement beyond the diffraction limit is crucial to the development of next-generation, super-resolution microscopy. STimulated Emission Depletion (STED) nanoscopy offers lateral super-resolution using a donut-beam depletion, but its axial resolution is still over 500 nm. Total internal reflection fluorescence microscopy is widely used for single-molecule localization, but its ability to detect molecules is limited …

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Video Talk by Roger Tsien: Fluorescent Protein Indicators

In this talk, Roger Tsien discusses how fluorescent proteins have been turned into indicators for a wide variety of biological molecules, including pH, ions, redox potential, and signaling molecules like phosphoinositides. The talk also covers reporters used to measure the activity of enzymes like kinases, phosphatases, and proteases. It covers both single proteins whose intensity …

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Wrinkled Giant Hyssop Leaf Protocol

IntroductionSpecies: Wrinkled Giant Hyssop (Agastache rugosa)Critical point drying of wrinkled giant hyssop leaf with subsequent gold coating and SEM analysis ProcedureSample Holder: Samples were inserted into the 22 mm cover slip holder Fixation and Dehydration 2.5% Glutaraldehyde in 0.1M Sodium Phosphate Buffer, pH 7.2 14 h 0.1M Sodium Phosphate Buffer, pH 7.2 3×10 min. Acetone …

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The Actin Cytoskeleton Modulates the Activation of iNKT Cells by Segregating CD1d Nanoclusters on Antigen-Presenting Cells

Invariant natural killer T (iNKT) cells recognize endogenous and exogenous lipid antigens presented in the context of CD1d molecules. The ability of iNKT cells to recognize endogenous antigens represents a distinct immune recognition strategy, which underscores the constitutive memory phenotype of iNKT cells and their activation during inflammatory conditions. However, the mechanisms regulating such “tonic” …

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