Month: June 2016

Individual Macromolecule Motion in a Crowded Living Cell

The application of the general model of the law of mass action itself, of the achievement of dynamic, statistical equilibria, has led to great successes in describing the theory of single-molecule biophysics and biochemistry based on individually and freely diffusing molecules in dilute liquid and crowded living cells. For example, anomalous diffusion is a general …

Individual Macromolecule Motion in a Crowded Living Cell Read More »

“The excellent red reflex, combined with the IOL guidance system, makes it possible for me to work quickly and precisely during refractive procedures.” | Learn & Share

Dr. Jung: These technologies are very important. In refractive surgery, it is important to correct the visual defect in the eye in the best possible way. For this reason, toric lens systems are a daily topic of discussion. Of course, patients ask, “What is best for me, my eyes and my life situation?” Some people …

“The excellent red reflex, combined with the IOL guidance system, makes it possible for me to work quickly and precisely during refractive procedures.” | Learn & Share Read More »

How to Manage Critical Points in Cataract or Refractive Surgery with IOL Positioning Software

The manual process: Time consuming and potentially unreliable ink marking Prior to surgery the toric lens orientation in the capsular bag has to be determined, ideally using a topographic device or other mediums capable of measuring the curvature values of the cornea. Calculations are then made by the pre-op device itself or manually by the …

How to Manage Critical Points in Cataract or Refractive Surgery with IOL Positioning Software Read More »

Two-Photon Excitation STED Microscopy with Time-Gated Detection

We report on a novel two-photon excitation stimulated emission depletion (2PE–STED) microscope based on time-gated detection. The time-gated detection allows for the effective silencing of the fluorophores using moderate stimulated emission beam intensity. This opens the possibility of implementing an efficient 2PE–STED microscope with a stimulated emission beam running in a continuous-wave. The continuous-wave stimulated …

Two-Photon Excitation STED Microscopy with Time-Gated Detection Read More »

The Oncogenic Triangle of HMGA2, LIN28B and IGF2BP1 Antagonizes Tumor-Suppressive Actions of the let-7 Family

The tumor-suppressive let-7 microRNA family targets various oncogene-encoding mRNAs. We identify the let-7 targets HMGA2, LIN28B and IGF2BP1 to form a let-7 antagonizing self-promoting oncogenic triangle. Surprisingly, 3′-end processing of IGF2BP1 mRNAs is unaltered in aggressive cancers and tumor-derived cells although IGF2BP1 synthesis was proposed to escape let-7 attack by APA-dependent (alternative polyadenylation) 3′ UTR …

The Oncogenic Triangle of HMGA2, LIN28B and IGF2BP1 Antagonizes Tumor-Suppressive Actions of the let-7 Family Read More »

TFG Promotes Organization of Transitional ER and Efficient Collagen Secretion

Collagen is the most abundant protein in the animal kingdom. It is of fundamental importance during development for cell differentiation and tissue morphogenesis as well as in pathological processes such as fibrosis and cancer cell migration. However, our understanding of the mechanisms of procollagen secretion remains limited. Here, we show that TFG organizes transitional ER …

TFG Promotes Organization of Transitional ER and Efficient Collagen Secretion Read More »