Application Note for Leica TIC 3X – High quality sample preparation of large area to investigate the sample in the SEM. For the mechanical preparation step diamond lapping foils of 9μm subsequently 2μm and finally 0.5μm grain size were used. It took about 1.5 hours. The sample was removed from the stub with a razor …
The application of the general model of the law of mass action itself, of the achievement of dynamic, statistical equilibria, has led to great successes in describing the theory of single-molecule biophysics and biochemistry based on individually and freely diffusing molecules in dilute liquid and crowded living cells. For example, anomalous diffusion is a general …
Individual Macromolecule Motion in a Crowded Living Cell Read More »
Dr. Jung: These technologies are very important. In refractive surgery, it is important to correct the visual defect in the eye in the best possible way. For this reason, toric lens systems are a daily topic of discussion. Of course, patients ask, “What is best for me, my eyes and my life situation?” Some people …
The manual process: Time consuming and potentially unreliable ink marking Prior to surgery the toric lens orientation in the capsular bag has to be determined, ideally using a topographic device or other mediums capable of measuring the curvature values of the cornea. Calculations are then made by the pre-op device itself or manually by the …
We report on a novel two-photon excitation stimulated emission depletion (2PE–STED) microscope based on time-gated detection. The time-gated detection allows for the effective silencing of the fluorophores using moderate stimulated emission beam intensity. This opens the possibility of implementing an efficient 2PE–STED microscope with a stimulated emission beam running in a continuous-wave. The continuous-wave stimulated …
Two-Photon Excitation STED Microscopy with Time-Gated Detection Read More »
The tumor-suppressive let-7 microRNA family targets various oncogene-encoding mRNAs. We identify the let-7 targets HMGA2, LIN28B and IGF2BP1 to form a let-7 antagonizing self-promoting oncogenic triangle. Surprisingly, 3′-end processing of IGF2BP1 mRNAs is unaltered in aggressive cancers and tumor-derived cells although IGF2BP1 synthesis was proposed to escape let-7 attack by APA-dependent (alternative polyadenylation) 3′ UTR …
A thin layer of latex paint was spread on a clean, scored, silicon chip. The sample was immediately plunge frozen in liquid ethane and transferred under LN2 to the Leica EM VCT100 loading station where it was placed in the customized sample holder. The holder was secured by the Leica EM VCT100 shuttle and transferred …
Application Note for Leica EM TIC 3X – Ion beam slope cutting is a method that can achieve cross sections of soft materials or material combinations consisting of hard and soft components. Source link
Collagen is the most abundant protein in the animal kingdom. It is of fundamental importance during development for cell differentiation and tissue morphogenesis as well as in pathological processes such as fibrosis and cancer cell migration. However, our understanding of the mechanisms of procollagen secretion remains limited. Here, we show that TFG organizes transitional ER …
TFG Promotes Organization of Transitional ER and Efficient Collagen Secretion Read More »
Dr. Raicov: The Leica microscope gives me the certainty that the treatment I have performed is correct. For prosthetic work, I can check the accuracy of the preparation. I have found the microscope to be a very useful diagnostic tool, too. Take sensitive teeth, for instance, in cases where the cause was difficult to identify, …
