Month: May 2016

Inspection of Multilayer Coating in the Automotive Industry

In which way have the demands on QC in the automotive industry changed with the development of new materials? Ruiz Balbas: Sustainability is the must in all areas of the automotive industry. This means we are now dealing with Non-CMR (Carcinogenic, Mutant and Reproductive) products with higher physic-chemical properties and at the same time going …

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Human Blood Cells Protocol

Introduction:Species: Human (Homo sapiens)Critical point drying of human blood with subsequent platinum / palladium coating and SEM analysis Procedure:Sample Holder:Samples were inserted into the 12 mm cover slip holder Preparation:Place 12 mm dia cover slip poly-L-lysine coated in a 12-wells cell culture plate. Add 1 ml 0.85% NaCl in each well to submerge each cover slip.Pipette gently …

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Image Gallery: Digital Microscopy Used in Microelectronics and Electronics Quality Control

Quality Control in the electronics and microelectronics industry is confronted with many challenges: A variety of different materials in a sample, some of them metallic and reflective, some matt; a variety of material layers and heights, most often very intricate and hard to discern. Users in microelectronics quality control seek to identify deviations from the …

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The Bimodally Expressed MicroRNA miR‐142 Gates Exit from Pluripotency

A stem cell’s decision to self‐renew or differentiate is thought to critically depend on signaling cues provided by its environment. It is unclear whether stem cells have the intrinsic capacity to control their responsiveness to environmental signals that can be fluctuating and noisy. Using a novel single‐cell microRNA activity reporter, we show that miR‐142 is …

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Quantifying the Resolution of a Leica SR GSD 3D Localization Microscopy System with 2D and 3D Nanorulers

The basic technology for constructing these defined structures on the nanoscale, invented in the year 2006 by Paul Rothemund, is the so called “DNA origami” technique [8]. A DNA origami structure is constructed out of a long (about 7,000 to 8,000 base pairs) single stranded DNA molecule the so called “scaffold strand” (Figure 2a). By adding ~200 …

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Workflows & Protocols: Laser Microdissection for Pathology and Cancer Research

Laser Microdissection was commercially developed* for more focused cancer research and mutation analysis. As some tumors need high purity to reveal their secret of the underlying mutation, a dedicated tool to extract microscopic amounts of pure tumor tissue – without any contaminating healthy cells – for unbiased and unmistakable downstream mutation analysis was re-created: laser …

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