Month: April 2016

Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing Agent

Super-resolution imaging deep inside tissues has been challenging, as it is extremely sensitive to light scattering and spherical aberrations. Here, we report an optimized optical clearing agent for high-resolution fluorescence imaging (SeeDB2). SeeDB2 matches the refractive indices of fixed tissues to that of immersion oil (1.518), thus minimizing both light scattering and spherical aberrations. During …

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Automotive Industry: How Suppliers and Auto Manufacturers Can Verify Parts Specifications Quickly and Easily

Checking specifications is critical: During the manufacture of auto parts, specifications must be met – whether by the auto parts supplier or the automobile manufacturer. It is important that the parts meet specifications as they are critical for maintaining the performance standards and safe operation of automobiles, trucks, and other vehicles during their lifetimes. However, …

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Six Features to Consider when Choosing a Dental Microscope

In combination with optical quality, illumination plays a key role in the brightness and color of the image that the dentist sees through the eyepiece. For natural color representation, light with daylight temperature of between 5,000 and 6,000 kelvin is optimal. Another factor to consider is the life-span of the light source as this …

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Definitions of Basic Technical Terms for Digital Microscope Cameras and Image Analysis

In every imaging system (i.e. monitor, print-out) any color depiction is based on a combination of single basic colors. Imaging methods are differentiated through additive and subtractive color mixing. For example, on a black monitor screen it is necessary to emit a certain type of light to yield a given color. In that case the …

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Improvement of Metallic Thin Films for HR-SEM by Using DC Magnetron Sputter Coater

To get a smooth and even surface, the sputter parameters which have the most influence on the film growth are systematically varied. Thickness, working distance, current and argon pressure are changed in a certain range. The effect on the morphology by changing these parameters is more precisely described in [6]. The most important parameter which …

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Characterizing the Evolution of Plant-Eating (Herbivorous) Mammals Using Dental Tribology

However, very little is known about the relationships between dental microwear textures and food mechanical properties. The question has been asked if dental microwear textures reflect more the global diet or rather the small amounts of “fallback” foods (when preferred foods are not available anymore), i.e., tough roots, hard seeds, tough and abrasive herbs. Also, …

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HyVolution – Super-Resolution Imaging with a Confocal Microscope

“Seeing is believing [10]” seems to be an obvious imperative for impartial observation in scientific experiments. However, the opposite is true. When we see something, objectivity ends behind the eye-lens. The retina, which is part of the complex and unpredictable computer called “brain”, already performs spatial and temporal computation of the incoming optical signal. The …

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