Month: December 2015

TEM Sample Preparation Made Easy – Prepare TEM Specimen by Broad Beam Argon Ion Milling

Quantitative and analytical analysis at high spatial resolution places stringent demands on the quality of the produced TEM specimens. Pristine and high-quality samples are indispensible for atomic resolution TEM analysis. In this application note a general procedure for obtaining cross-sectional and plan-view TEM specimens using the Leica EM RES102 ion milling system is outlined. The …

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Super-Resolution Microscopy of the Synaptic Active Zone

Brain function relies on accurate information transfer at chemical synapses. At the presynaptic active zone (AZ) a variety of specialized proteins are assembled to complex architectures, which set the basis for speed, precision and plasticity of synaptic transmission. Calcium channels are pivotal for the initiation of excitation-secretion coupling and, correspondingly, capture a central position at …

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Lytic Immune Synapse Function Requires Filamentous Actin Deconstruction by Coronin 1A

Lytic immune effector function depends upon directed secretion of cytolytic granules at the immunological synapse (IS) and requires dynamic rearrangement of filamentous (F)-actin. Coronin 1A (Coro1A) is the hematopoietic-specific member of the Coronin family of actin regulators that promote F-actin disassembly. Here, we show that Coro1A is required for natural killer (NK) cell cytotoxic function …

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Workflows & Protocols: How to Isolate Individual Chromosomes with Laser Microdissection

Beside tissue preparation and dissection for RNA downstream analysis, DNA analysis (e.g. for mutation detection) is desired. As a single cell commonly contains two pairs of chromosomes, the isolation of single chromosomes is particularly important for chromosome-specific diseases as well as for the understanding of gene structures and contribution of genes in different species. With …

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