Month: September 2015

“The Leica Digital Light Sheet Module – a Clever Example of Thinking Out of the Box” | Learn & Share

Bram van den Broek is a postdoctoral fellow at the Netherlands cancer institute in Amsterdam where he supports the advanced microscopy techniques in the laboratory of Kees Jalink. His main project is developing a fast single image frequency domain FLIM technique. But he is also involved in many other research projects, such as super-resolution imaging …

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Skin analysis system BeautyExplorer™ / Stories / Sony Design / Sony

Exploring the future of health and beauty Everyday health and beauty should be easier and smarter – that’s the idea behind the BeautyExplorer system, which enables informed choices from advanced skin analysis. Welcome to a more beautiful future, as designed by Sony. Envisioning everyday health and beauty Trendsetting and exciting ideas come from a clear …

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Novel Microscopy-Based Screening Method Reveals Regulators of Contact-Dependent Intercellular Transfer

Microscopy in the context of biomedical research is demanding new tools to automatically detect and capture objects of interest. The few extant packages addressing this need, however, have enjoyed limited uptake due to complexity of use and installation. To overcome these drawbacks, we developed iMSRC, which combines ease of use and installation with high flexibility …

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Probes that FIT RNA

We have been developing new tools based on fluorogenic forced intercalation (FIT) probes for RNA detection quantification and interference in biological samples. The probes contain a thiazole orange (TO) dye which is introduced at a position normally occupied by a nucleobase. Upon duplex formation with target nucleic acids, the TO dye base surrogate increases its …

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Co-Orientation: Quantifying Simultaneous Co-Localization and Orientational Alignment of Filaments in Light Microscopy

Co-localization analysis is a widely used tool to seek evidence for functional interactions between molecules in different color channels in microscopic images. Here we extend the basic co-localization analysis by including the orientations of the structures on which the molecules reside. We refer to the combination of co-localization of molecules and orientational alignment of the …

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A Brief History of Light Microscopy – From the Medieval Reading Stone to Super-Resolution

The highest microscope magnifications for quite a time to come were achieved by the Dutchman Antonie van Leeuwenhoek, who lived from 1632 to 1723. The cloth merchant, for whom microscopy was only a hobby at first, managed to magnify objects more than 200 times with his microscopes. Instead of working with compound microscopes like Hooke, …

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Progressive Glucose Stimulation of Islet Beta Cells Reveals a Transition From Segregated to Integrated Modular Functional Connectivity Patterns

Collective beta cell activity in islets of Langerhans is critical for the supply of insulin within an organism. Even though individual beta cells are intrinsically heterogeneous, the presence of intercellular coupling mechanisms ensures coordinated activity and a well-regulated exocytosis of insulin. In order to get a detailed insight into the functional organization of the syncytium, …

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High-Resolution audio headphones h.ear™ / Stories / Sony Design / Sony

Letting listeners find their own style, in new classics What kind of headphones would satisfy individualists who don’t compromise on style? Our answers remain focused on audio quality and couldn’t be simpler. Stylish Hi-Res headphones that are as simple as a blank canvas. Listen with your own signature style For those with a clear sense …

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Localization of HDAC1 Using Super-Resolution STED Microscopy

IHC Sample tissue was fixated by formalin fixed paraffin embedding. Protein detection was performed using primary antibodies at 5 µg/mL (HDAC5 and HDAC10) or 1:500 (HDAC1) for 1 h at room temperature. Secondary antibody: peroxidase rabbit secondary antibody (Rockland, p/n 611-103-122) at 1:10,000 for 45 min at room temperature. Proteins were visualized as precipitated red or purple signal …

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