Month: July 2015

First Bilateral Hand Transplant in a Child

Surgeons at The Children’s Hospital of Philadelphia (CHOP) joined with colleagues from Penn Medicine recently to complete the world’s first bilateral hand transplant on a child. Earlier this month, the surgical team successfully transplanted donor hands and forearms onto 8-year-old Zion Harvey who, several years earlier, had undergone amputation of his hands and feet and …

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Cortical Actin Networks Induce Spatio-temporal Confinement of Phospholipids in the Plasma Membrane – A Minimally Invasive Investigation by STED-FCS

Important discoveries in the last decades have changed our view of the plasma membrane organisation. Specifically, the cortical cytoskeleton has emerged as a key modulator of the lateral diffusion of membrane proteins. Cytoskeleton-dependent compartmentalised lipid diffusion has been proposed, but this concept remains controversial because this phenomenon has thus far only been observed with artefact-prone …

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Using Telecentric Optical Systems to Optimize Forensic Image Accuracy and Reproducibility

When the first compound microscopes were invented in 1590, scientists marveled at their new ability to see tiny objects and features that were previously invisible to the eye and therefore seemingly nonexistent. Ever since then, the study of these miniscule details has brought science into a forensic world once ruled by intuition and deduction. The …

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A Lipid Bound Actin Meshwork Organizes Liquid Phase Separation in Model Membranes

The eukaryotic cell membrane is connected to a dense actin rich cortex. We present FCS and STED experiments showing that dense membrane bound actin networks have severe influence on lipid phase separation. Our results reveal a mechanism how cells may prevent macroscopic demixing of their membrane components, while at the same time regulate the local …

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Sony Design: MAKING MODERN Event Report / Events / News / Sony

Event Report Commemorating the Publication ofSony Design: MAKING MODERN To celebrate the publication of Sony Design: MAKING MODERN, an exhibition was organized at the Sony Building in Ginza. A pre-event talk was held on April 28. Joining head of Sony Design Yutaka Hasegawa to discuss the spirit of Sony Design and enduring appeal of Sony …

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Workflows & Protocols: How to Use a Leica Laser Microdissection System and Qiagen Kits for Successful RNA Analysis

All samples (A–D) with different treatments have almost the same quantity, only sample C (fixed, stained and picked directly from slide) shows higher quantity than positive control (sample A). No influence from EtOH fixation, staining or Leica LMD dissection detectable. Cryo-storage of slide in a Parafilm-sealed 50 ml Falcon tube at –80 °C overnight, gently thawed step-wise for …

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Multi-protein Assemblies Underlie the Mesoscale Organization of the Plasma Membrane

Most proteins have uneven distributions in the plasma membrane. Broadly speaking, this may be caused by mechanisms specific to each protein, or may be a consequence of a general pattern that affects the distribution of all membrane proteins. The latter hypothesis has been difficult to test in the past. Here, we introduce several approaches based …

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Critical-point Drying for the Preparation of Biological Samples for MicroCT Analysis

X-ray micro-computed tomography (micro-CT) is a routinely applied non-invasive technique for the investigation of the internal anatomy and morphology of organisms. As a result of a micro-CT scan a stack of grey-scale images is generated from a series of projections taken at defined angles during sample rotation. Since several years the number of lab-based micro-CT …

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Transmission Electron Microscopy Sample Preparation Protocols for the Ultrastructural Study of Cysts of Free-living Protozoa

Cysts of free-living protozoa have an impact on the ecology and epidemiology of bacteria because they may act as a transmission vector or shelter the bacteria against hash environmental conditions. Detection and localization of intracystic bacteria and examination of the en- and excystment dynamics is a major challenge because no detailed protocols for ultrastructural analysis …

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