Month: June 2015

Transnational Telepathology Consultations Using a Basic Digital Microscope: Experience in the Italy-Slovenjia INTERREG Project

BackgroundIn recent years, a number of technological advancements started to modify the long standing appearance and functionalities of traditional optical microscopes used in Pathology and other medical fields. In fact, at present many new tools for microscopical visualization exist that are based on digital imaging, robotization, and remote communication. Such tools are typically adopted in …

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Using Telecentric Optical Systems to Optimize Industrial Image Accuracy and Reproducibility

With so many errors hidden in the optics of standard microscopes, it may seem impossible to reliably inspect anything requiring quantitative reporting. However, careful consideration of a microscope’s optical design can avoid this problem. For example, some microscopes are available with telecentric optics that eliminate or dramatically reduce inaccuracies and loss of reproducibility caused by …

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Abstracts of the 5th European Super-Resolution User-Club Meeting

Imre Gaspar, Developmental Biology Unit, EMBL, Heidelberg, Germany In vast majority of the studied animal species, body axes are determined already during oogenesis through localization and localized translation of messenger RNA molecules. These mRNAs contain specific structures (cis-acting elements) usually – but not limited to – in their 3’ untranslated region which recruit specific protein …

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iMSRC: Converting a Standard Automated Microscope into an Intelligent Screening Platform

Microscopy in the context of biomedical research is demanding new tools to automatically detect and capture objects of interest. The few extant packages addressing this need, however, have enjoyed limited uptake due to complexity of use and installation. To overcome these drawbacks, we developed iMSRC, which combines ease of use and installation with high flexibility …

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Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury

In this video article we describe a zebrafish model of AKI using gentamicin as the nephrotoxicant. The technique consists of intravenous microinjections on 2 dpf zebrafish. This technique represents an efficient and rapid method to deliver soluble substances into the bloodstream of zebrafish larvae, allowing for the injection of 15–20 fish per hour. In addition …

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High Quality Sample Preparation for EBSD Analysis by Broad Ion Beam Milling

Electron Backscatter Diffraction technique (EBSD) is known as a “surface” technique because electron diffraction is generated within a few tens of nanometers of the sample surface. Therefore, the specimen surface should be exempt of any damages in order to produce EBSD patterns. Here, we present a successful and efficient EBSD sample polishing of two very …

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Improving Axial Resolution in Confocal Microscopy with New High Refractive Index Mounting Media

Resolution, high signal intensity and elevated signal to noise ratio (SNR) are key issues for biologists who aim at studying the localisation of biological structures at the cellular and subcellular levels using confocal microscopy. The resolution required to separate sub-cellular biological structures is often near to the resolving power of the microscope. When optimally used, …

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Correlative In-Resin Super-Resolution and Electron Microscopy Using Standard Fluorescent Proteins

We introduce a method for correlative in-resin super-resolution fluorescence and electron microscopy (EM) of biological structures in mammalian culture cells. Cryo-fixed resin embedded samples offer superior structural preservation, performing in-resin super-resolution, however, remains a challenge. We identified key aspects of the sample preparation procedure of high pressure freezing, freeze substitution and resin embedding that are …

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