Month: May 2015

Preparation of Small Disk-shaped Samples for Capacitance and Pyroelectric Current Measurements

where C is the sample capacitance to be measured, ε0 is the vacuum dielectric permittivity, α is the cooling rate in the pyroelectric current measurement and Ip(T) is the pyroelectric current measured at a given temperature, T. Precise measurements require well-defined shapes and a high degree of parallelism of layer-shaped samples. This is difficult to …

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Correlative Light and Electron Microscopy – Get Your Free CLEM e-Book for Download

The urge to go deeper into the microscopic world has led researchers to combine the versatility of the light microscopy (LM) with the resolution power of the electron microscope (EM) to produce Correlative Light and Electron Microscopy (CLEM). CLEM’s most significant and powerful characteristic for cell biology research is an ability to study the same …

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What You Always Wanted to Know About Digital Microscopy, but Never Got Around to Asking

A very obvious limitation of digital microscopes, compared to e.g. stereo or compound microscopes, is the need for a power connection. Because there are no eyepieces, the image of the sample for digital microscopes is always displayed on a monitor. Thus, the need for at least 1 power cable. In general, digital microscopes also need a …

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Switching Roles: The Functional Plasticity of Adult Tissue Stem Cells

Adult organisms have to adapt to survive, and the same is true for their tissues. Rates and types of cell production must be rapidly and reversibly adjusted to meet tissue demands in response to both local and systemic challenges. Recent work reveals how stem cell (SC) populations meet these requirements by switching between functional states …

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Detailed Morphological Characterisation of Hendra Virus Infection of Different Cell Types Using Super-Resolution and Conventional Imaging

BackgroundHendra virus (HeV) is a pleomorphic virus belonging to the Paramyxovirus family. Our long-term aim is to understand the process of assembly of HeV virions. As a first step, we sought to determine the most appropriate cell culture system with which to study this process, and then to use this model to define the morphology …

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Confocal and Digital Light Sheet Imaging

3. The conjunction Both paradigms have been introduced in biomedical research and routine, serving for many new insights and understandings of biological concepts and diseases, and of the construction and function of cellular components. In terms of instrumentation, the two ways are significantly different, the orthogonal illumination was treated as a separate part of the …

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Multilayered Polyelectrolyte Microcapsules: Interaction with the Enzyme Cytochrome C Oxidase

Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time. The interaction of polyelectrolyte microcapsules, fabricated by electrostatic layer-by-layer assembly, with cytochrome c oxidase molecules was investigated. We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and …

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