Month: March 2015

Negative Space – Audio design concept / Stories / Sony Design / Sony

Showing what’s behind premium audio Listening to music on premium audio products is a treat.To tempt a wider audience, we have developed a new design language. Satisfying more listeners with impeccable sound Casual MP3 or AAC listening is now very common, fueled by the rise of smartphones and mobile devices, as fewer seek out the …

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Interview with Dr. Gertrude Bunt and Prof. Fred S. Wouters on the FOM 2015

What are the greatest challenges for you as organizer in staging a conference like the FOM in Göttingen? Wouters: Unfortunately, Göttingen doesn’t have a conference center. So the first major challenge was to find a suitable venue for the FOM at all. The experience of past years enabled an accurate estimate of the conditions that …

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Four questions for Professor Stefan Hell on the subject of FOM 2015

What does it mean to you that the FOM is being held in Göttingen this year? I’m pleased that Göttingen is the venue for this year’s FOM because Göttingen is a stronghold of microsopy – all the way from basic research to applications in life sciences and commercial manufacture. Göttingen is one of the ‘constant …

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Total RNA Isolation after Laser-capture Microdissection of Human Cervical Squamous Epithelial Cells from Fresh Frozen Tissue

As most tissue specimens contain a mixture of different cell types including epithelial cells, stromal cells and immune cells, selection of the cells of interest is of utmost importance for the accurate determination of gene/microRNA expression. Laser capture microdissection enables the researcher to obtain homogeneous ultrapure cell selections from heterogeneous starting material. The following protocol …

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Cryo CLEM – the Combination of Cryo Fluorescence Microscopy with Cryo Electron Microscopy

Limitations of fluorescence and electron microscopy When studying biological events within a cell or at the level of molecular interactions, scientists are often limited by the spatial resolution of FM. So-called “super-resolution” methods go some way towards overcoming this limitation to allow the localisation of individual fluorescent labels with a resolution reaching tens of nanometres. …

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Five Advantages of Inverted Over Upright Microscopes in Industrial Applications

With an inverted microscope, you simply place your sample on the stage, focus onto the surface once and image it. Finished. The sample stays focused for all magnifications and further samples of the same sort are in focus alike. With an upright microscope the workflow spans more tasks operators need to perform: You need …

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Pathways to Optical STED Microscopy

Optical far-field microscopy such as confocal fluorescence microscopy is a very popular technique for investigating the living cell. Unfortunately, its spatial resolution is limited to around 200 nm, impeding the imaging of small molecular assemblies. Recent decades have seen the development of optical nanoscopy, optical far-field microscopy with a spatial resolution down to molecular scales. STED …

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Video Talk by Daniel Axelrod: Total Internal Reflection Fluorescence (TIRF) Microscopy

About iBiology iBiology (www.ibiology.org) is a collection of open-access online videos by scientists about research and discoveries that cover a wide variety of life science subjects. iBiology has produced over 80 videos by the world’s leading scientists including seminars about research in fields from ecology to biophysics, a 65-video course on light microscopy, and over …

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