Month: February 2015

In recent years, carbon nanotubes, with high tensile strength and electrical conductivity, have been used in polymer composites to improve their mechanical and electrical properties. As a result, nanotube-polymer composites have been explored as a new class of materials. In particular, single-walled carbon nanotubes (SWNTs) have outstanding charge transport and are becoming more and more …

Nanoscale or Microscale Structures Formed in Polymers Containing Nanotubes Greatly Enhance the Electrical Conductivity: Potential Applications for Photovoltaic Devices Read More »

High speed scanning is necessary to image rapidly changing biological processes. With traditional scanning techniques, imaging speed is limited by the number of fluorophores in a specimen. And, rapid acquisition often comes at the cost of image resolution. This video demonstrates how the Leica Microsystems’ Tandem Scanner works and why this two-in-one system helps you …

Video: High Speed Scanning – With two Scanners in one System Read More »

Cryo-electron microscopy (cryo-EM) allows for the observation of high resolution structures of biological samples as close to the native state as possible. In contrast to conventional EM methods no chemical fixatives, such as glutaraldehyde or osmiumtetroxide, or contrast enhancing heavy metal stains, such as uranyl acetate, are used. This minimises the risk of fixation and …

New Insights into Cilia and Flagella by Cryo-EM Read More »

GSDIM microscopy is a widefield super-resolution technique based on the localization of fluorophores with nanometer precision. With its help a lateral resolution of down to 20 nm can be achieved, whereas the new 3D feature even shrinks axial resolution to 50 nm. Here we provide a collection of publications around that super-resolution microscopy method also …

GSDIM Publication List Read More »

The glass-supported planar lipid bilayer system has been utilized in a variety of disciplines. One of the most useful applications of this technique has been in the study of immunological synapse formation, due to the ability of the glass-supported planar lipid bilayers to mimic the surface of a target cell while forming a horizontal interface. …

Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer Read More »

A well established usage case for cryo-TEM is three-dimensional reconstruction of isolated macromolecules, virus particles, or filaments. On one hand, these approaches are based on averaging of repetitive structures – either due to numerous identical molecules, repetitive patterns on a filament, or symmetries, to reduce the noise inherent to cryo-TEM. On the other hand, different …

Immersion Freezing for Cryo-Transmission Electron Microscopy: Applications Read More »

A key issue is producing vitreous ice by sufficiently fast cooling, but also maintaining the ice in this amorphous state: the latter requires that the sample is kept constantly below recrystallisation temperature. This can be ensured by keeping the grid below LN2 or in a cold N2 gas atmosphere and  pre-cooling all tools required for …

Immersion Freezing for Cryo-Transmission Electron Microscopy: Fundamentals Read More »

The lateral window technique is a method of maxillary sinus floor elevation which enables implant placement in the maxillary posterior region where the maxillary sinus is enlarged and/or the alveolar bone height is deficient. When creating a lateral window, great care should be taken not to perforate the maxillary sinus membrane. While carefully examining the …

Video: Implant Microsurgery – Microscope-­aided Maxillary Sinus Floor Elevation, Lateral Window Technique Read More »