Month: December 2014

Identification of Molecular Pathways Facilitating Glioma Cell Invasion In Situ

Gliomas are mostly incurable secondary to their diffuse infiltrative nature. Thus, specific therapeutic targeting of invasive glioma cells is an attractive concept. As cells exit the tumor mass and infiltrate brain parenchyma, they closely interact with a changing micro-environmental landscape that sustains tumor cell invasion. In this study, we used a unique microarray profiling approach …

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JC Virus Inclusions in Progressive Multifocal Leukoencephalopathy: Scaffolding Promyelocytic Leukemia Nuclear Bodies Grow With Cell Cycle Transition Through an S-to-G2–Like State in Enlarging Oligodendrocyte Nuclei

In progressive multifocal leukoencephalopathy, JC virus–infected oligodendroglia display 2 distinct patterns of intranuclear viral inclusions: full inclusions in which progeny virions are present throughout enlarged nuclei and dot-shaped inclusions in which virions are clustered in subnuclear domains termed “promyelocytic leukemia nuclear bodies” (PML-NBs). Promyelocytic leukemia nuclear bodies may serve a scaffolding role in viral progeny …

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Recovery of Recombinant Antibody is Affected by Endogenous Protein Interaction in Maize

Seeds provide a useful and versatile platform for the production of recombinant proteins and their numerous advantages have been often discussed [1]. Among seeds, cereal crops offer additional advantages such as high yield and well-stablished agricultural infrastructure which allows easy up and down scaling in response to demand. Moreover, cereal endosperm is naturally adapted for protein …

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Cleanliness Analysis in Relation to Particulate Contamination

Determining product cleanliness Evaluation of cleanliness and cleaning processes The assessment of cleanliness for particulate contamination, both before and after the product component undergoes a cleaning process, can be done by 2 approaches: a direct method or an indirect method [1]. A direct method involves the direct examination of surfaces without extraction or transfer of particles, …

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Video Tutorials: Filling and Assembling of Different Carriers for High-Pressure Freezing

This system is the most frequently used and it exists in two variations: flat specimen system for 3 mm carriers and for 6 mm carriers. It is suitable for an array of applications utilizing the freezing of different cultured cell types, bacteria, algae, animal and plant tissues, etc. Samples frozen in the flat specimen carriers are …

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How to Choose the Right Confocal Microscope for Your Lab?

Confocal microscopy has come a very long way since its invention more than a half-century ago. Today, with novel technology driven by leading imaging companies, it has become the standard for fluorescence microscopy. Choosing the right confocal microscope for your specific research requires the appropriate mix of features related to resolution, sensitivity, and speed. Confocal …

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