Jean-Luc Vonesch is head of the imaging facility at the Institute of Genetics and Molecular and Cellular Biology (IGBMC), Strasburg, France. 23 years ago he was the founder of this facility which nowadays serves more than 850 scientists distributed among 47 working groups. Accordingly the technical spectrum at the imaging facility is very brought because …
Gliomas are mostly incurable secondary to their diffuse infiltrative nature. Thus, specific therapeutic targeting of invasive glioma cells is an attractive concept. As cells exit the tumor mass and infiltrate brain parenchyma, they closely interact with a changing micro-environmental landscape that sustains tumor cell invasion. In this study, we used a unique microarray profiling approach …
Identification of Molecular Pathways Facilitating Glioma Cell Invasion In Situ Read More »
In progressive multifocal leukoencephalopathy, JC virus–infected oligodendroglia display 2 distinct patterns of intranuclear viral inclusions: full inclusions in which progeny virions are present throughout enlarged nuclei and dot-shaped inclusions in which virions are clustered in subnuclear domains termed “promyelocytic leukemia nuclear bodies” (PML-NBs). Promyelocytic leukemia nuclear bodies may serve a scaffolding role in viral progeny …
Seeds provide a useful and versatile platform for the production of recombinant proteins and their numerous advantages have been often discussed [1]. Among seeds, cereal crops offer additional advantages such as high yield and well-stablished agricultural infrastructure which allows easy up and down scaling in response to demand. Moreover, cereal endosperm is naturally adapted for protein …
Recovery of Recombinant Antibody is Affected by Endogenous Protein Interaction in Maize Read More »
Determining product cleanliness Evaluation of cleanliness and cleaning processes The assessment of cleanliness for particulate contamination, both before and after the product component undergoes a cleaning process, can be done by 2 approaches: a direct method or an indirect method [1]. A direct method involves the direct examination of surfaces without extraction or transfer of particles, …
Cleanliness Analysis in Relation to Particulate Contamination Read More »
This system is the most frequently used and it exists in two variations: flat specimen system for 3 mm carriers and for 6 mm carriers. It is suitable for an array of applications utilizing the freezing of different cultured cell types, bacteria, algae, animal and plant tissues, etc. Samples frozen in the flat specimen carriers are …
Video Tutorials: Filling and Assembling of Different Carriers for High-Pressure Freezing Read More »
Confocal microscopy has come a very long way since its invention more than a half-century ago. Today, with novel technology driven by leading imaging companies, it has become the standard for fluorescence microscopy. Choosing the right confocal microscope for your specific research requires the appropriate mix of features related to resolution, sensitivity, and speed. Confocal …
How to Choose the Right Confocal Microscope for Your Lab? Read More »
