Month: August 2014

Spectral and Structural Comparison Between Bright and Dim Green Fluorescent Proteins in Amphioxus

The cephalochordate Amphioxus naturally co-expresses fluorescent proteins (FPs) with different brightness, which thus offers the rare opportunity to identify FP molecular feature/s that are associated with greater/lower intensity of fluorescence. Here, we describe the spectral and structural characteristics of green FP (bfloGFPa1) with perfect (100 %) quantum efficiency yielding to unprecedentedly-high brightness, and compare them to …

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Sony Design

Through the power of design, we envision new paradigms, give meaning to ideas, and engage globally ― a process that resonates with a diversity of values, bringing new standards into being. As we continue to explore the limitless possibilities of design, we will work to create new value standards with people across the globe. Source …

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Correlated Optical and Isotopic Nanoscopy

Molecular processes in living cells can best be monitored by high-resolution microscopy techniques. Although groundbreaking technical innovations in the field of microscopy have been made in the past, frontiers still exist. Prof. Dr. Silvio O. Rizzoli and his team of the Göttingen DFG Research Center and Cluster of Excellence for Nanoscale Microscopy and Molecular Physiology …

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Single-Cell Phenotyping within Transparent Intact Tissue through Whole-Body Clearing

Understanding the structure-function relationships at cellular, circuit, and organ-wide scale requires 3D anatomical and phenotypical maps, currently unavailable for many organs across species. At the root of this knowledge gap is the absence of a method that enables whole-organ imaging. Herein, we present techniques for tissue clearing in which whole organs and bodies are rendered …

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Video Talk by Jeff Lichtman: Resolution in Microscopy – Wave Optics and the Diffraction Limit

About iBiology iBiology is a collection of open-access online videos by scientists about research and discoveries that cover a wide variety of life science subjects. iBiology has produced over 80 videos by the world’s leading scientists including seminars about research in fields from ecology to biophysics, a 65-video course on light microscopy, and over 90 …

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STED Microscopy of Living Cells – New Frontiers in Membrane and Neurobiology

Recent developments in fluorescence far-field microscopy such as STED microscopy have accomplished observation of the living cell with a spatial resolution far below the diffraction limit. Here, we briefly review the current approaches to super-resolution optical microscopy and present the implementation of STED microscopy for novel insights into live cell mechanisms, with a focus on …

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125 Years of Comparison Microscopy – From the

Because the “camera lucida” generated an additional magnification, however, the part images of the two microscopes not only differed in magnification, but also in intensity. In view of these shortcomings, lnostranzeff gave the St. Petersburg University Mechanicus H. Frantzen specifications for making a comparison chamber in 1885. This could be mounted on two adjacent microscopes …

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Abstracts of the 4th European Super-Resolution User-Club Meeting

“People really interact during the sessions – this is  really important and helps to create a nice atmosphere. I look forward to participating in the 3rd meeting”, said Giuseppe Vicidomini, one of the speakers at this year’s meeting, who specializes in gated confocal super-resolution techniques at the IIT, Genoa, Italy. “The meeting enables users of …

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