Month: July 2014

The Basic Principles of Laser Microdissection

Laser Microdissection (LMD) is a technique for isolating specific and pure targets from microscopic heterogeneous samples for downstream analysis (DNA, RNA & proteins); based on microscopic imaging and utilizing a laser. In contrast to other systems which use a fixed laserfocus for dissection, Leica Microsystems’ LMD systems guide the laserfocus for dissection. This unique …

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Glucose-Regulated Protein 78 (Grp78) Confers Chemoresistance to Tumor Endothelial Cells under Acidic Stress

This study was designed to investigate the activation of the unfolded protein response (UPR) in tumor associated endothelial cells (TECs) and its association with chemoresistance during acidic pH stress.Materials and methods: Endothelial cells from human oral squamous cell carcinomas (OSCC) were excised by laser capture microdissection (LCM) followed by analysis of UPR markers (Grp78, ATF4 …

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Super-resolution Molecular and Functional Imaging of Nanoscale Architectures in Life and Materials Science

Super-resolution (SR) fluorescence microscopy has been revolutionizing the way in which we investigate the structures, dynamics, and functions of a wide range of nanoscale systems. In this review, I describe the current state of various SR fluorescence microscopy techniques along with the latest developments of fluorophores and labeling for the SR microscopy. I discuss the …

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Super-Resolution Microscopy Helped to Create the First 3D Model of a Synapse

“This 3D model of a synapse opens a new world for neuroscientists,” says Prof. Rizzoli, senior author of the publication. Particularly the abundance and distribution of the building blocks have long been terra incognita, an undiscovered land. The model presented by Prof. Rizzoli and his team now shows several hundreds of thousands of individual proteins …

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