Natural killer cells form tightly regulated, finely tuned immunological synapses (IS) in order to lyse virally infected or tumorigenic cells. Dynamic actin reorganization is critical to the function of NK cells and the formation of the IS. Imaging of F-actin at the synapse has traditionally utilized confocal microscopy, however the diffraction limit of light restricts …
M. Parkinson research A characteristic sign of M. Parkinson is the deterioration of dopaminergic neurons in the mid-brain, specifically in the substantia nigra (SN, black substance). Different causes and forms of this disease have been identified. In the case of genetic familial forms, for example, it has been possible to identify various genes that have …
Stimulated emission depletion (STED) microscopy is a prominent approach of super-resolution optical microscopy, which allows cellular imaging with so far unprecedented unlimited spatial resolution. The introduction of time-gated detection in STED microscopy significantly reduces the (instantaneous) intensity required to obtain sub-diffraction spatial resolution. If the time-gating is combined with a STED beam operating in continuous …
We received 14 awards in Red Dot Award: Product Design 2014. Two of the award-winning products, XperiaTM Z Ultra and DSC-QX100/QX10, received Red Dot: Best of the Best. Source link
Trypanosoma brucei cultured in suspension were centrifuged at 300 g for 5 min. After removal of the supernatant, the 100 μl pellet was resuspended in 1 volume 20 % BSA in ddH2O. The suspension was injected into a 15.8 mm U-tube using a 20 μl pipet with a cut tip until it was completely filled, displacing all air bubbles. The filled tubes were …
At the center of the circular ditch, there is now a reconstructed six-meter high burial mound which housed two warriors’ graves with numerous burial objects. While recovering the finds, the excavation team made an important decision, setting a new precedent for future restoration projects. Frank Bodis, head of the hessenArchäologie restoration workshop in Wiesbaden, recalls: …
Myths Under the Microscope: The Restoration of Celtic Finds Read More »
DNA methylation plays a crucial role in development through inheritable gene silencing. Plants possess three types of DNA methyltransferases (MTases), namely Methyltransferase (MET), Chromomethylase (CMT) and Domains Rearranged Methyltransferase (DRM), which maintain methylation at CG, CHG and CHH sites. DNA MTases have not been studied in legumes so far. Here, we report the identification and …
What makes CLARITY so exciting is the fact, that you can investigate whatever you want in one single brain. Protein complexes, gene expression profiling, neuronal circuits – you don’t have to decide what you want to see. Look at all of it, one after the other. The nanoporous hydrogel mesh is permeable for macromolecules and …
In endocrine cells within islets of Langerhans calcium ions couple cell stimulation to hormone secretion. Since the advent of modern fluorimetry, numerous in vitro studies employing primarily isolated mouse islets have investigated the effects of various secretagogues on cytoplasmic calcium, predominantly in insulin-secreting beta cells. Due to technical limitations, insights of these studies are inherently …
We propose a network representation of electrically coupled beta cells in islets of Langerhans. Beta cells are functionally connected on the basis of correlations between calcium dynamics of individual cells, obtained by means of confocal laser-scanning calcium imaging in islets from acute mouse pancreas tissue slices. Obtained functional networks are analyzed in the light of …
Functional Connectivity in Islets of Langerhans from Mouse Pancreas Tissue Slices Read More »
