Month: September 2013

Brief Introduction to Glass Knifemaking for Electron and Light Microscope Applications

Scoring and breaking principles Producing good glass knives routinely depends on a supply of reproducible squares, an accurately positioned score and controlled pressure precisely applied to make the break. As a general rule, the knife edge is straighter and the counterpiece (knife shoulder) is small when the fracture occurs close to the corner (long score). …

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Investing in Ergonomically Designed Microscope Workplaces Pays Off

Basically, it’s important to gain awareness for ergonomic behavior. This includes knowing the potential of the available instrumentation and making use of it. Often, the microscope can be optimized by using a different tube or eyepiece; then you have made a change for the better. It’s a good idea to try it out with a …

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Three-Dimensional Super-Resolution GSDIM Microscopy

Cellular compartmentalization is maintained by targeted protein trafficking in vesicular structures along cytoskeletal tracks. A detailed characterization of these cellular components is essential for an understanding of cellular functions. Super-resolution imaging methods based on single molecule localization have begun to bring these small structures into focus. Source link

Towards Real-time Image Deconvolution: Application to Confocal and STED Microscopy

Although deconvolution can improve the quality of any type of microscope, the high computational time required has so far limited its massive spreading. Here we demonstrate the ability of the scaled-gradient-projection (SGP) method to provide accelerated versions of the most used algorithms in microscopy. To achieve further increases in efficiency, we also consider implementations on …

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