Month: July 2013

Safe High Pressure Freezing of Infectious Microorganisms

Checking influence of high pressure freezing to viability of bacteria (Figure 11, dark blue line) and if the platelet sealed in the pod is leaky after pressurization and cooling (Figure 11, light blue line). Same procedure as described in Figures 4–6, but before checking bacterial growth the sample was high pressure frozen. Source link

A Precise and Rapid Mapping Protocol for Correlative Light and Electron Microscopy of small invertebrate organisms

Background information: CLEM (correlative live cell and electronmicroscopy) seeks to bridge the data acquired with different imaging strategies, typically between light microscopy and electron microscopy. It has been successfully applied in cell cultures, although its use in multicellular systems is hampered by difficulties in locating the ROI (region of interest). Results: We developed a CLEM …

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Direct In Vivo Evidence for Tumor Propagation by Glioblastoma Cancer Stem Cells

High-grade gliomas (World Health Organization grade III anaplastic astrocytoma and grade IV glioblastoma multiforme), the most prevalent primary malignant brain tumors, display a cellular hierarchy with self-renewing, tumorigenic cancer stem cells (CSCs) at the apex. While the CSC hypothesis has been an attractive model to describe many aspects of tumor behavior, it remains controversial due …

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Carbon Thickness Evaluation in Electron Microscopy

However, this method applied for carbon layers has some downsides. The heat and light generated from carbon evaporation can influence the frequency of the quartz. Only after some time can a stabilised value give a reasonable thickness measurement. Figures 1 and 2 illustrate how the evaporation process influences the frequency of the quartz crystal over time. …

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Abstracts of the 3rd European Super-Resolution User-Club Meeting

Alberto Diaspro, Italian Institute of Technology, Genoa, Italy It is well established that for fluoresence, the most popular imaging mode in optical microscopy, the diffraction barrier no longer provides an unsurpassable limitation for resolution and localization accuracy. Furthermore, the terms “super-resolution” and the earlier coined “optical nanoscopy”, have been implemented in real far field optical …

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STED Nanoscopy with Time-Gated Detection: Theoretical and Experimental Aspects

In a stimulated emission depletion (STED) microscope the region in which fluorescence markers can emit spontaneously shrinks with continued STED beam action after a singular excitation event. This fact has been recently used to substantially improve the effective spatial resolution in STED nanoscopy using time-gated detection, pulsed excitation and continuous wave (CW) STED beams. We …

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Ergonomically Designed Surgical Microscopes Support Performance

Operations not only demand maximum concentration of the surgeon but also maximum physical fitness. Physiotherapist John Ludescher explains how the body can be given optimal support to perform at its best. The Austrian qualified as physiotherapist in Switzerland and has had his own practice for nine years. He is the author of the book “Aufrecht …

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