Month: June 2013

Spectral Detection – How to Define the Spectral Bands that Collect Probe-specific Emission

An entirely different approach for selecting the desired band from the entire spectrum is the use of mechanical devices, folded with the spectrum. If the light is passed through a dispersive element (prism or grating), the components are spread spatially depending on their energy. The energy of photons corresponds with the color. In a simple …

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Multiple Microscopy Modes in a Single Sweep with Supercontinuum White Light

The white light laser source, merged with hybrid detectors in photon counting mode and true spectral detection offers a series of different imaging modes from a single instrument. The continuously tunable excitation, combined with different pulse-frequencies covers all requirements for FLIM. Gated detection allows to separate fluorescent species that exert identical emission color, but differ …

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Brief Introduction to High-Pressure Freezing

Water is the most abundant cellular constituent and therefore important for preserving cellular ultra-structure. Currently the only way to fix cellular constituents without introducing significant structural alterations is by cryo-fixation. There are currently two common methods employed; plunge freezing and high pressure freezing. Cryo-fixation has two distinct advantages over chemical fixation. It is achieved within …

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