An entirely different approach for selecting the desired band from the entire spectrum is the use of mechanical devices, folded with the spectrum. If the light is passed through a dispersive element (prism or grating), the components are spread spatially depending on their energy. The energy of photons corresponds with the color. In a simple …
The white light laser source, merged with hybrid detectors in photon counting mode and true spectral detection offers a series of different imaging modes from a single instrument. The continuously tunable excitation, combined with different pulse-frequencies covers all requirements for FLIM. Gated detection allows to separate fluorescent species that exert identical emission color, but differ …
Multiple Microscopy Modes in a Single Sweep with Supercontinuum White Light Read More »
Water is the most abundant cellular constituent and therefore important for preserving cellular ultra-structure. Currently the only way to fix cellular constituents without introducing significant structural alterations is by cryo-fixation. There are currently two common methods employed; plunge freezing and high pressure freezing. Cryo-fixation has two distinct advantages over chemical fixation. It is achieved within …
