As shown above, the number of edges in a regular polygon has no effect on the transmission of the focus signal. There is no reason to assume that this is equally true for defocus signals. It seems that circular, square and hexagonal pinholes are identical in performance. For classical, filter-based confocal microscopes this might be …
Fluorescence Recovery After Photobleaching (FRAP) has been considered the most widely applied method for observing translational diffusion processes of macromolecules. The resulting information can be used to determine kinetic properties, like the diffusion coefficient, mobile fraction, and transport rate of the fluorescently labeled molecules. FRAP employs irradiation of a fluorophore with a short laser pulse. …
The precise measurement of surface structures and topography is the key thing during the production, control and development in many sectors of industry or research. Because very often there is not possible to use some contact methods for performing this task new optical methods based on interferometry and confocal technology started to be available for …
As early as 1912, the so-called “Strahlenstich” method was used to focus light for micromanipulation in biology and medicine, marking the beginning of experiments that eventually led to laser microdissection (Tschachotin, 1912). However, it was 1962 before Bessis and Bereiter-Hahn et al. irradiated samples with ruby lasers (Bessis) and UV laser beams (Bereiter-Hahn) to cut …
The biologist sees her cooperation in the project as an extremely enriching experience for her personally. “It was the first time I was allowed to work on mummies, and it was very interesting. The teamwork with my colleagues was incredibly valuable and highly enjoyable – we learnt a lot from each other. I always love …
In the Catacombs of the Capuchin Monastery in Palermo Read More »
