Month: May 2013

Step by Step Guide for FRAP Experiments

Fluorescence Recovery After Photobleaching (FRAP) has been considered the most widely applied method for observing translational diffusion processes of macromolecules. The resulting information can be used to determine kinetic properties, like the diffusion coefficient, mobile fraction, and transport rate of the fluorescently labeled molecules. FRAP employs irradiation of a fluorophore with a short laser pulse. …

Step by Step Guide for FRAP Experiments Read More »

Automated Digital 3D Topography Measurement with the Leica DCM 3D Dual Core Measuring Microscope

The precise measurement of surface structures and topography is the key thing during the production, control and development in many sectors of industry or research. Because very often there is not possible to use some contact methods for performing this task new optical methods based on interferometry and confocal technology started to be available for …

Automated Digital 3D Topography Measurement with the Leica DCM 3D Dual Core Measuring Microscope Read More »