Month: April 2013

Immersion Freezing of Suspended Particles and Cells for Cryo-Electron Microscopy

Immersion freezing of thin aqueous specimens is an essential preparation technique for cryo-transmission electron microscopy (cryo-TEM), aiming to preserve fragile biological structures such as molecules and cells in their hydrated environment for a close-to-native visualization. For successful experiments, vitreous ice must be produced, surface contamination must be avoided, and, most important, the natural state of …

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Videos on the Leica History (German only)

Ernst Leitz Optische Werke Wetzlar YouTube video by Deutsches Filminstitut – DIF e.V. Germany before 1932Director: Oskar Barnack Brought to you by “filmarchives online”, the web gateway to European film archives:http://www.filmarchives-online.euWith the support of the MEDIA Plus programme of the European Community. A portrait of the Leitz factory. Following a view of Wetzlar and the …

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Imaging Enzymes at Work

For the understanding of functions of proteins in biological and pathological processes, reporter molecules such as fluorescent proteins have become indispensable tools for visualizing the location of these proteins in intact animals, tissues, and cells. For enzymes, imaging their activity also provides information on their function or functions, which does not necessarily correlate with their …

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Image Cytometry

Microscopy-based imaging is booming and the need for tools to retrieve quantitative data from images is urgent. This book provides simple but reliable tools to generate valid quantitative gene expression data, at the mRNA, protein and activity level, from microscopic images in relation to structures in cells, tissues and organs in 2D and 3D. Volumes, …

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