This article gives an overview of two different types of superresolution techniques. Stimulated emission depletion (STED) microscopy is a versatile and fast method that is based on point scanning microscopy – usually an extension of a confocal microscope. Ground state depletion imaging ( Source link
This article summarizes the development and differences in design and functionality of confocal technology as far as spectral properties are concerned:from classical filter-based excitation and emission color selection to fully flexible spectral excitation and emission tuning without planoptical elements (“filter-free”). All three major components: light source with excitation color selection, beam splitting for incident illumination …
Modern biomedical research is currently dominated by imaging and measuring with optical microscopes. One branch of the microscopy technology is confocal microscopy. For correlation purposes, multiparameter fluorescence imaging is particularly of unique interest. This article is concerned with the spectral performance of the various modules in a confocal point-scanning microscope (“True Confocal System”), and how …
The ability of the nervous system to learn and respond to the environment reflects an underlying capability of neurons to dynamically alter the number, type, and strengths of their connections. These connections, called synapses, are highly organized sites of contact between postsynaptic neurons and presynaptic terminals. The specialized synaptic membrane, contains a large variety of …
Capturing Neurotransmitter Receptors and Ion Channels Read More »
Using the sequencing information provided by other research groups, Andreas Hejnol and his team then examine, for instance, the genes involved in the formation of the nervous system of both the fly and the human – two very distantly related creatures. They then compare these observations with the function of these genes in more closely …
The most frequently used method for post-staining is a twostep procedure of staining with uranyl acetate (UA), followed by lead citrate. Uranyl acetate is used as an aqueous or alcoholic solution with a pH for the saturated solution in the range of 3.5 to 4.0. The addition of alcohol, especially methanol, increases the solubility (Hayat, …
Perusing Alternatives for Automated Staining of TEM Thin Sections Read More »
The first MAS Cathodoluminescence Topical Conference was hosted October 24–28, 2011 by the National Institute of Standards and Technology (NIST) in Gaithersburg, MD. This conference was sponsored by the Microbeam Analysis Society (MAS), and was co-sponsored by the Australian Microbeam Analysis Society (AMAS). The four day program included a pre-conference tutorial targeted for the CL …
