Month: January 2013

University of Wollongong Electron Microscopy Centre

To address a number of performance issues related to electron microscopy, the University of Wollongong has constructed a purpose built electron microscopy centre that was completed in July 2011, and purchased a comprehensive suite of specimen preparation equipment that is now operational for materials and life sciences. The centre will house up to 7 electron …

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Widefield Calcium Imaging with Calcium Indicator Fura2

The Ca2+ unbound form of Fura2 gets excited at 380 nm and the Ca2+ bound form at 340 nm. The emitted light is measured at around 510 nm. The fluorescence intensity increases at 340 nm with increasing Ca2+ concentration and decreases at 387 nm for the unbound form. There are some techniques to introduce the fluorescent Ca2+ indicator into cells, like …

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Mitochondrial DNA Molecules are Packaged Individually

The evolutionary precursors of today’s mitochondria gave up their independent existence as bacteria about 2 billion years ago in order to live on symbiotically inside other cells (endosymbiosis). Meanwhile they supply most of the energy for nearly all the cells in our body: Most of the energy in nutrients is utilized inside the mitochondria by …

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100 Years of Binoculars and Quantitative Microscopy

One hundred years ago, in 1913, the Optische Werke Ernst Leitz in Wetzlar, predecessor of Leica Microsystems CMS GmbH, made two inventions that were to blaze the trail for modern microscopy: the binocular tube and the integrating stage for quantitative microscopy. Throughout the 400-year history of microscopy, microscopists have always wanted to be able to …

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