Month: December 2012

Light Sheet Fluorescence Microscopy: Beyond the Flatlands

Light Sheet Fluorescence Microscopy (LISH-M) is a true fluorescence optical sectioning technique, first described by Heinrich Siedentopf in 1902 under the name of Ultramicroscopy. Light sheet microscopy utilises a plane of light to optically section samples. This allows deep imaging within transparent tissues and whole organisms. As tissues are exposed to only a thin plane …

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Dry Ultrathin Sectioning Combined With High Pressure Freezing

Cell culture models of bone mineralization: UMR106-01 cells do it faster, are temporally synchronized, and produce more mineral We have used cultured UMR106-01 osteoblastic cells to investigate the process of bone mineralization. UMR106-01 cells as well as primary calvarial bone cells assemble spherical extracellular supramolecular protein-lipid complexes, termed biomineralization foci (BMF), in which the first …

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Tubulin Modifications Affect Monolayer Formation and Apical Trafficking in Epithelial Cells

To analyze the apical and basolateral transport processes in polarized cells it was necessary to check the arrangement of different tubulin modifications by using immunofluorescence microscopy. In MDCK cell cysts (grown in Matrigel) detyrosinated and tyrosinated microtubules were equally distributed along the apical-basolateral axis (Figure 1A). Neither detyr- nor tyr-tubulin preferentially concentrates at one of …

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