Comparison of the RNA Quality of Native Tissue Samples with Tissue Samples after UV Laser Microdissection
To compare untreated tissue samples with fixed and stained tissue samples, three cryo-asserved murine brains of known RNA integrity (RIN) were used. After cryo-asservation at –80 °C, these were transferred to a cryomicrotome and incubated at –35 °C for 45 min. Then the tissue was equilibrated at –18 °C for 30 min and cut into 12 µm sections at –18 °C by …