Month: July 2012

Digital Camera Technologies for Scientific Bio-Imaging

This four-part series of articles published in Microscopy and Analysis covers the factors to consider in choosing a camera among CCD, EMCCD, and scientific-grade CMOS camera technologies for biological imaging applications. The differences among the sensor architectures and the impact of parameters such as pixel size, noise, and QE on signal-to-noise performance, image quality, and …

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CARS Microscopy: Imaging Characteristic Vibrational Contrast of Molecules

The chemical bonds in molecules can shake, bend and rattle. They make these motions at particular rates or frequencies. These frequencies are so particular that we can identify what kind of chemical bond is rattling by tuning into their particular vibrational frequencies. For instance, many organic molecules contain bonds between carbon and hydrogen atoms, and …

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Widefield Super-Resolution with GSDIM

Great advancements in biology have been possible by using fluorescence microscopy. So far, the resolution of the images was limited due to physical constraints. In the past couple of years, new methods evolved circumventing these limitations and bringing fluorescence microscopy to a new level of resolution, boosting the possibilities in science with fluorescence microscopes. Ground …

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