Month: June 2012

White Light Laser

How should the perfect light source for confocal microscopes in biomedical applications perform? It should have sufficient intensity, tunable color for simultaneous excitation of a series of samples. Furthermore, it should be a pulsed source for lifetime fluorescence experiments. Such a source has been invented: the white light laser. It consists of a high-energy pulsed …

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Fluorescent Dyes

In fluorescence microscopy it is often reasonable to stain cell compartments like lysosomes or endosomes and organelles like mitochondria. For this purpose there is a palette of specific dyes available which will be mentioned in this section. One well known way to observe mitochondria is the utilization of MitoTrackerĀ®. This is a cell permeable dye …

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