Month: February 2012

Live-cell Imaging Techniques

The understanding of complex and/or fast cellular dynamics is an important step for exploring biological processes. Therefore, today’s life science research is increasingly focusing on dynamic processes like cell migration, morphological changes of cells, organs or whole animals and physiological (e.g. changes of intracellular ion composition) events in living specimens in real time. One approach …

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How to Study Protein Recruitment to DNA Lesions by a Combination of UV Laser and White Light Laser

Cell cultivation Human osteosarcoma U2OS cells stably expressing GFP-BMI1 originated from the laboratory of Prof. Maarten Van Lohuizen, The Netherlands Cancer Institute, Antoni van Leeuwenhoek Ziekenhuis, Amsterdam, The Netherlands. We obtained these cells from the laboratory of Assoc. Prof. Dušan Cmarko, Charles University in Prague. Mouse 3T3 cells were a generous gift from Dr. Paul …

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Region-Specific Gene Expression in Adult Mouse CNS Tissues

Preparation of retina sections. Whole eyes of n = 3 C57BL/6 adult (two month old) mice were dissected, flash frozen in tissue freezing medium and stored at –80 °C. Sections (15 µm) were prepared with a Leica CM1850UV cryostat and placed on Leica PEN-membrane slides. Sections were fixed in cold 75 % ethanol (2 min), washed twice in RNAse-free …

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Fluorescent Proteins – From the Beginnings to the Nobel Prize

All the effort researches put into the discovery, understanding and enhancement of fluorescing proteins lead to a huge range of applications in life sciences. GFP and its variants opened the door for scientists to watch f.e. metastasis or angiogenesis in living organisms. Moreover the use of fluorescent multicolored neurons (Brainbow) will help to understand complex …

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