Subcellular Localization of AKT and Tubulin using Super-Resolution Microscopy
Conditions for STED nanoscopy: A431 cells (ATCC CRL-1555) were incubated at 37 °C with 5 % CO2 in DMEM (Invitrogen, MO) with 10 % FBS (Rockland, PA) and Pen Strep (Invitrogen, USA), in 75 cm2 Corning CellBind cell culture flasks (Sigma, MO) unless otherwise noted. At 70 to 80 % confluence cells were detached using TrypLE (Invitrogen, MO), and then …
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