Month: June 2011

An Introduction to Laser Microdissection

Laser microdissection offers a precise and contamination-free solution for the isolation and selection of single cells or tissue. The tissue samples can be embedded, sectioned and stained according to conventional methods of preparation. Paraffin sections, frozen sections, smear preparations, chromosome specimens and cell cultures are all suitable for laser microdissection. The area selected for dissection …

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Applications of Laser Microdissection

An examination of genetic information using molecular biological and biochemical methods is termed gene expression analysis. This provides both quantitative and qualitative information on genetic activity. Microarray technology has become increasingly widespread in genome analysis, diagnostics and gene expression analysis. By means of these so-called gene chips it is possible to examine the expression of …

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Lasers for Confocal

A light source that is suitable for confocal illumination needs to fulfill a range of requirements: brightness, stability, focusability and appropriate wavelength – besides moderate cost, low energy consumption, and tolerable heat and noise generation. Focusability is the most important parameter, as the target is to focus the light to a diffraction-limited spot. Extended light …

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Nanoscopy in a Living Multicellular Organism Expressing GFP

We report superresolution fluorescence microscopy in an intact living organism, namely Caenorhabditis elegans nematodes expressing green fluorescent protein (GFP)-fusion proteins. We also superresolve, by stimulated emission depletion (STED) microscopy, living cultured cells, demonstrating that STED microscopy with GFP can be widely applied. STED with GFP can be performed with both pulsed and continuous-wave lasers spanning …

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Phase Contrast

Phase contrast microscopy visualizes differences in the optical path length of a specimen. The optical path length is related to the specimen’s thickness and the refractive index. Cellular structures like plasma membranes and organelles have a profound impact on the optical path length. As many cells (especially in cell cultures) have a flat and regular …

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