Month: April 2011

A mTurquoise-Based cAMP Sensor for Both FLIM and Ratiometric Read-Out Has Improved Dynamic Range

FRET-based sensors for cyclic Adenosine Mono Phosphate (cAMP) have revolutionized the way in which this important intracellular messenger is studied. The currently prevailing sensors consist of the cAMP-binding protein Epac1, sandwiched between suitable donor- and acceptor fluorescent proteins (FPs). Through a conformational change in Epac1, alterations in cellular cAMP levels lead to a change in …

A mTurquoise-Based cAMP Sensor for Both FLIM and Ratiometric Read-Out Has Improved Dynamic Range Read More »

Fluorescence in Microscopy

Fluorescence microscopy is widely used and offers great specificity. Various techniques make it possible to address different problems and even to circumvent the diffraction limit that was described by Ernst Abbe. The localization of a molecule species can be determined with a co-staining of organelles, e.g. the cytoskeleton or membranes. Confocal laser scanning microscopy …

Fluorescence in Microscopy Read More »

Users Report on the Relevance of Laser Microdissection for Their Research Results

Prof. Monica Gotta from the Department of Genetic Medicine and Development at the University of Geneva uses the LMD for the study of asymmetric cell division, which allows the generation of cell diversity during development and stem cell renewal: “Establishment of cell polarity and positioning of the mitotic spindle are essential prerequisites for asymmetric cell …

Users Report on the Relevance of Laser Microdissection for Their Research Results Read More »

Good Vibrations

In recent years, new molecular imaging techniques, such as coherent anti-Stokes Raman scattering microscopy (CARS), have been developed for rapid vibrational imaging of living cells. Source link

TIRF Microscopy of the Apical Membrane of Polarized Epithelial Cells

Figure 2a shows a comparison of conventional epi­fluorescence images and the image of various fluorophores on the apical membrane domain of MDCK cells made by TIRFM. Here, a predominantly apical membrane protein, the neurotrophin receptor (p75), was studied in comparison with its apical sorting receptor, galectin-3. The two proteins were labelled with fluorescence proteins, p75 …

TIRF Microscopy of the Apical Membrane of Polarized Epithelial Cells Read More »

Mapping Billions of Synapses with Microscopy and Mathematics

Microscopy and mathematics We had been using an upright epifluorescence microscope, acquiring dual channel, three micron-thick z-sections in order to reconstruct 3D immunofluorescent images. The challenge for us was to maintain our optical sections at varying depths in the tissue section to match the optimal plane of labeling. To do this we needed an autofocus …

Mapping Billions of Synapses with Microscopy and Mathematics Read More »