4D Photoactivation of pa-GFP in Living Cells Using Two-Photon Excitation Laser Scanning Microscopy

Green fluorescent protein (GFP) from Aqueorea Victoria [1] and its multicolored variations on the theme are among the most routinely fluorescent tracers used for biological visualization [2]. Interest has grown in more precise localization studies of protein activity and movement within a cell and we could say that a new revolution started with the advent …

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