Fluorescence Recovery after Photobleaching with the Leica TCS SP2

Among all photobleaching experiments which have been described, fluorescence recovery after photobleaching (FRAP) is the most popular. It employs irradiation of a fluorophore in a living sample with a short laser pulse to degrade it and thereby abolish fluorescence followed by time-resolved image recording of the sample. If the fluorophore (or a so-called mobile fraction …

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